Error message

  • Notice: Undefined index: custom_date_format in date_formatter_format() (line 447 of /data/crossleylab.unsw.edu.au/www/sites/all/modules/contrib/date/date.module).
  • Notice: Undefined index: formatted_timezone in theme_date_display_combination() (line 139 of /data/crossleylab.unsw.edu.au/www/sites/all/modules/contrib/date/date.theme).
  • Notice: Undefined index: formatted_time in theme_date_display_combination() (line 145 of /data/crossleylab.unsw.edu.au/www/sites/all/modules/contrib/date/date.theme).
  • Notice: Undefined index: formatted_time in theme_date_display_combination() (line 147 of /data/crossleylab.unsw.edu.au/www/sites/all/modules/contrib/date/date.theme).
Krüppel-like factor 3 (KLF3/BKLF) is required for widespread repression of the inflammatory modulator galectin-3 (Lgals3). J Biol Chem. 2016 May 24. pii: jbc.M116.715748. [Epub ahead of print] PMID: 27226561
Author: 

Knights AJ, Yik JJ, Jusoh HM, Norton LJ, Funnell AP, Pearson RC, Bell-Anderson KS, Crossley M, Quinlan KG.

Key Summary: 
J Biol Chem. 2016 May 24. pii: jbc.M116.715748. [Epub ahead of print]
Krüppel-like factor 3 (KLF3/BKLF) is required for widespread repression of the inflammatory modulator galectin-3 (Lgals3).
Knights AJ1, Yik JJ1, Jusoh HM2, Norton LJ1, Funnell AP1, Pearson RC1, Bell-Anderson KS2, Crossley M1, Quinlan KG3.
Author information
 
Abstract
The Lgals3 gene encodes a multifunctional β-galactoside-binding protein, galectin-3. Galectin-3 has been implicated in a broad range of biological processes, from chemotaxis and inflammation to fibrosis and apoptosis. The role of galectin-3 as a modulator of inflammation has been studied intensively and recent evidence suggests that it may serve as a protective factor in obesity and other metabolic disorders. Despite considerable interest in galectin-3, little is known about its physiological regulation at the transcriptional level. Here, using knockout mice, chromatin immunoprecipitations, cellular and molecular analyses we show that the zinc finger transcription factor Krüppel-like Factor 3 (KLF3) directly represses galectin-3 transcription. We find that galectin-3 is broadly up-regulated in KLF3-deficient mouse tissues, that KLF3 occupies regulatory regions of the Lgals3 gene, and that KLF3 directly binds its cognate elements (CACCC boxes) in the galectin-3 promoter and represses its activation in cellular assays. We also provide mechanistic insight into the regulation of Lgals3, demonstrating that C-terminal binding protein (CtBP) is required to drive optimal KLF3-mediated silencing. These findings help to enhance our understanding of how expression of the inflammatory modulator galectin-3 is controlled, opening up avenues for potential therapeutic interventions in the future.
Copyright © 2016, The American Society for Biochemistry and Molecular Biology.